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KMID : 0355820230440010075
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Korean Journal of Oral Anatomy
2023 Volume.44 No. 1 p.75 ~ p.84
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The effect of scaling on odontogenic ameloblast-associated protein (ODAM) expression in the gingival crevicular fluid
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Cho A-Ram
Bae Hyun-Sook
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Abstract
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This study aimed to evaluate periodontal status by comparing changes in odontogenic ameloblast-associated protein (ODAM) after scaling interventions utilizing samples of gingival crevicular fluid (GCF). For GCF analysis, a total of 76 GCF samples, collected from 12 to 20 teeth, from 17 patients with gingivitis and periodontitis before and after scaling were collected. The patients were selected based on inclusion and exclusion criteria The total GCF levels were quantified by enzyme-linked immunosorbent assay (ELISA). After GCF collection, PD, PI, CI, and mSBI were measured on the same teeth using a periodontal probe Furthermore, the post-scaling intervention evaluation was performed after a 4-week healing period. A Wilcoxon signed ranks test analysis was conducted to determine the differences in clinical parameters (PD, PI, CI, mSBI) and ODAM in gingival crevicular fluid before and after scaling intervention. PD, PI, CI, and mSBI were all significantly different before and after scaling (p < 0.001). There was a significant difference in ODAM concentration between pre- and post-scaling (p < 0.05). There was also a significant difference in the level of ODAM expression before and after scaling in periodontitis patients with a PD of 4 mm or more than in gingivitis patients with a PD of less than 3 mm. The present study confirmed changes in ODAM within GCF after scaling. ODAM is a biomarker that reflects the active state of periodontitis and has high sensitivity and specificity for the extent of the disease, making it useful for accurately diagnosing the early stages of the disease and evaluating the effectiveness of treatment. Our results confirmed changes in ODAM within GCF after scaling. In the future, further studies are warranted. If the mechanism of ODAM detection in the GCF of patients with gingivitis is confirmed and significant differences in ODAM concentrations in the GCF of subjects with moderate and severe periodontal disease with PD ≥ 4 mm, ODAM may be a potential objective indicator of periodontal disease activity. If GCF can be used as a biomarker to assess and monitor periodontal disease activities and periodontal changes following treatment, periodontal disease prevention and management may be further improved.
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KEYWORD
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Odontogenic ameloblast-associated protein(ODAM), Biomarker, Dental Scaling, Gingival crevicular fluid, Periodontitis
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